The amino acid sequence of T4 phage lysozyme. 3. Peptic digestion.
نویسندگان
چکیده
A total of 47 peptides have been isolated from a chymotrypsin digest of T4 phage lysozyme by column chromatography, followed by paper electrophoresis or paper chromatography (or both). Their complete or partial amino acid sequences were determined. Utilizing information obtained from 16 overlapping peptides and seven incompletely digested tryptic peptides, it has been possible to assemble 26 nonoverlapping tryptic peptides to five unique fragments of this enzyme. Chymotryptic peptides also accounted for a total of 164 amino acid residues of T4 phage lysozyme.
منابع مشابه
The Amino Acid Sequence of T4 Phage Lysozyme
A total of 47 peptides have been isolated from a chymotrypsin digest of T4 phage lysozyme by column chromatography, followed by paper electrophoresis or paper chromatography (or both). Their complete or partial amino acid sequences were determined. Utilizing information obtained from 16 overlapping peptides and seven incompletely digested tryptic peptides, it has been possible to assemble 26 no...
متن کاملA Genetic and Structural Analysis of P22 Lysozyme: A Thesis
GENETIC AND STRUCTURAL ANAYLYSIS OF P22 LYSOZYME P22 lysozyme , encoded by gene 19, is an essential phage protein responsible for hydrolyzing the bacterial cell wall during lytic infection. P22 lysozyme is related to T4 lysozyme in its mode of action, substrate specificities , and in its structure. Gene 19 was located on the phage genome , subcloned and then sequenced. Lysozyme was produced in ...
متن کاملThe Disulfide Bonds of Egg White Lysozyme (muramidase).
The availability of the amino acid sequence of egg white lysoeyme makes it possible to identify the pairing of the 8 half-cystine residues that give rise to the four disulfide bonds present in the native protein (1,2). In establishing the positions of the disulfide bonds in insulin (3, 4) and ribonuclease (5, 6) it was shown that disulfide interchange, which occurs under certain experimental co...
متن کاملPurification of Bacteriophage T4 Lysozyme*
The lysozyme of bacteriophage T4 was purified to apparent homogeneity from lysates of the phage grown on Escherichia coli. The enzyme is a single polypeptide chain of molecular weight 19,000, with a single NH&erminal methionine residue and a single COOH-terminal leuciue residue. The amino acid composition of the protein was determined. The phage lysozyme exhibits a much greater specific activit...
متن کاملThe Amino Acid Sequence of Egg White Lysozyme.
The separation and characterization of peptic and chymotryptic peptides of egg white lysozyme by phosphocellulose chromatography has been reported (1). In addition, a group of 18 different tryptic peptides of lysozyme, the total composition of which approximates that of the whole protein, has been characterized (2). This communication reports further studies that define a unique sequence for th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of biological chemistry
دوره 245 14 شماره
صفحات -
تاریخ انتشار 1970